TY - JOUR
T1 - An evaluation of bacterial contamination of barriers used in periapical tissue regeneration
T2 - Part 2-Bacterial penetration
AU - Sharma, Priya
AU - Mickel, André K.
AU - Chogle, Sami
AU - Sharma, Prem
AU - Han, Yiping W.
AU - Jones, Jefferson J.
PY - 2008/3/1
Y1 - 2008/3/1
N2 - Objective: To compare the relative penetration of Prevotella melaninogenica and Enterococcus faecalis through 3 guided tissue regeneration membranes: Atrlsorb, Lambone, and OsseoQuest. It was hypothesized that OsseoQuest would show increased bacterial penetration when compared to Lambone and Atrisorb. Method and Materials: Centrifuge tubes containing trypticase soy broth were sealed with circular sections of membranes and placed in test tubes containing culture media. The bacterial penetration was assessed by passage of bacteria from the outer tube culture media to the inner centrifuge tube media through the membrane. After incubation for 4 and 48 hours, the media from the outer and inner tubes were compared for bacterial count. Results: P melaninogenica exhibited 91% penetration for Lambone in 2 days, while OsseoQuest displayed 87% penetration with E faecalis In the same time. Atrisorb displayed a minimal penetration with both bacteria (2%). Conclusion: Atrisorb displayed the least bacterial penetration, which may be attributed to membrane structure, chemical configuration, hydrophobicity, and porosity of tested membranes.
AB - Objective: To compare the relative penetration of Prevotella melaninogenica and Enterococcus faecalis through 3 guided tissue regeneration membranes: Atrlsorb, Lambone, and OsseoQuest. It was hypothesized that OsseoQuest would show increased bacterial penetration when compared to Lambone and Atrisorb. Method and Materials: Centrifuge tubes containing trypticase soy broth were sealed with circular sections of membranes and placed in test tubes containing culture media. The bacterial penetration was assessed by passage of bacteria from the outer tube culture media to the inner centrifuge tube media through the membrane. After incubation for 4 and 48 hours, the media from the outer and inner tubes were compared for bacterial count. Results: P melaninogenica exhibited 91% penetration for Lambone in 2 days, while OsseoQuest displayed 87% penetration with E faecalis In the same time. Atrisorb displayed a minimal penetration with both bacteria (2%). Conclusion: Atrisorb displayed the least bacterial penetration, which may be attributed to membrane structure, chemical configuration, hydrophobicity, and porosity of tested membranes.
KW - Bacterial penetration
KW - Barrier membranes
KW - Enterococcus faecalis
KW - Guided tissue regeneration membrane
KW - Prevotella melaninogenica
KW - Surgery
UR - http://www.scopus.com/inward/record.url?scp=38949134765&partnerID=8YFLogxK
M3 - Article
C2 - 18618039
AN - SCOPUS:38949134765
VL - 39
SP - 237
EP - 242
JO - Quintessence International
JF - Quintessence International
SN - 0033-6572
IS - 3
ER -