Buoyant density and molecular weight calculations on an Apple II microcomputer from equilibrium banding experiment on analytical ultracentrifuge

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Buoyant density is one of the non-destructive measurements of viruses, nucleic acids etc. which help in characterizing these entities in terms of their chemical composition and physical conformation. In recent years equilibrium sedimentation in CsCl buoyant density gradient has been proved to be a powerful tool for genome characterization. The method enables one to obtain information such as the absolute and the relative buoyant density of the material and its molecular weight. This communication describes a program in BASIC which calculates these parameters from the raw data obtained directly from the experiment. The program is fully interactive, user-oriented, and written keeping in view biologists and biochemists as the main users. The program has a built-in option to calculate either the absolute or the relative buoyant density and the molecular weight.

Original languageEnglish
Pages (from-to)189-193
Number of pages5
JournalComputer Methods and Programs in Biomedicine
Volume24
Issue number3
DOIs
Publication statusPublished - 1 Jan 1987

Keywords

  • Apple II microcomputer
  • Buoyant density
  • CsCl-density gradient
  • Equilibrium banding
  • Isopycnic gradient centrifugation
  • Sedimentation equilibrium

Fingerprint Dive into the research topics of 'Buoyant density and molecular weight calculations on an Apple II microcomputer from equilibrium banding experiment on analytical ultracentrifuge'. Together they form a unique fingerprint.

Cite this