TY - JOUR
T1 - Colorectal carcinomas from Middle East. Molecular and tissue microarray analysis of genomic instability pathways
AU - Bavi, Prashant P.
AU - Abubaker, Jehad
AU - Jehan, Zeenath D.
AU - Al-Jomah, Naif A.
AU - Siraj, Abdul K.
AU - Al-Harbi, Sayer R.
AU - Atizado, Valerie L.
AU - Abduljabbar, Alaa S.
AU - Alhomoud, Samar J.
AU - Ashari, Luai H.
AU - Al-Dayel, Fouad H.
AU - Uddin, Shahab
AU - Al-Kuraya, Khawla S.
AU - Alsanea, Nasser A.
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Objectives: To evaluate the overall incidence of microsatellite instability (MSI), hereditary non polyposis colorectal cancer, and tumor supressor gene (TP53) mutations in Saudi colorectal carcinomas. Methods: We studied the MSI pathway in Saudi colorectal cancers (CRC) from 179 unselected patients using 2 methods, MSI by polymerase chain reaction, and immunohistochemistry detection of mutL homologs 1 and mutS homologs 2 proteins. The TP53 mutations were studied by sequencing exons 5, 6, 7, and 8. Results: Of the 150 colorectal carcinomas analysed for MSI, 16% of the tumors showed high level instability (MSI-H), 19.3% had low-level instability (MSI-L) and the ramaining 64% tumors were stable. Survival of the MSI-H group was better as compared to the MSI-L or microsatellite stable group (p=0.0217). In the MSI-H group, 48% were familial MSI tumors, which could be attributable to the high incidence of consaguinity in the Saudi population. The TP53 mutations were found in 24% of the cases studied. Conclusions: A high proportion of familial MSI cases and a lower incidence of TP53 mutations are some of the hallmarks of the Saudi colorectal carcinomas, which need to be explored further.
AB - Objectives: To evaluate the overall incidence of microsatellite instability (MSI), hereditary non polyposis colorectal cancer, and tumor supressor gene (TP53) mutations in Saudi colorectal carcinomas. Methods: We studied the MSI pathway in Saudi colorectal cancers (CRC) from 179 unselected patients using 2 methods, MSI by polymerase chain reaction, and immunohistochemistry detection of mutL homologs 1 and mutS homologs 2 proteins. The TP53 mutations were studied by sequencing exons 5, 6, 7, and 8. Results: Of the 150 colorectal carcinomas analysed for MSI, 16% of the tumors showed high level instability (MSI-H), 19.3% had low-level instability (MSI-L) and the ramaining 64% tumors were stable. Survival of the MSI-H group was better as compared to the MSI-L or microsatellite stable group (p=0.0217). In the MSI-H group, 48% were familial MSI tumors, which could be attributable to the high incidence of consaguinity in the Saudi population. The TP53 mutations were found in 24% of the cases studied. Conclusions: A high proportion of familial MSI cases and a lower incidence of TP53 mutations are some of the hallmarks of the Saudi colorectal carcinomas, which need to be explored further.
UR - http://www.scopus.com/inward/record.url?scp=40949096092&partnerID=8YFLogxK
M3 - Article
C2 - 18176677
AN - SCOPUS:40949096092
VL - 29
SP - 75
EP - 80
JO - Saudi Medical Journal
JF - Saudi Medical Journal
SN - 0379-5284
IS - 1
ER -