Effect of epigallocatechin gallate on uncoupling protein 2 in acute liver injury

Mohammad H. Jamal, Hamad Mohamed Ali Yaseen, Ali Dashti, Jasim Al-Abbad, Husain Dashti, Chako Mathew, Waleed Al-Ali, Sami Asfar

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Abstract

Background: The aim of this study was to investigate the effect of epigallocatechin gallate (EGCG) on uncoupling protein 2 regulation in an acute liver injury-animal model. Methods: Twenty seven male Wistar rats were divided into three groups: control group (n = 9), TAA group (n = 9): acute liver injury was induced by the intraperitoneal injection of thioacetamide (200 mg/kg) and EGCG/TAA (n = 9 rats): Epigallocatechin gallate was given two weeks prior to the induction of acute liver injury by thioacetamide. The levels of uncoupling protein 2, CRP, TNF-α and interleukins (IL) 6 and 18 were analyzed in the liver using PCR analysis. Results: Q-PCR analysis showed that the genetic expression of UCP2, TNF-α and CRP in the EGCG/TAA group was the least in comparison to other groups (P ≤ 0.005). The IL-6 and IL-18 were upregulated after induction of acute liver injury, but this upregulation was significantly less in the group that received epigallocatechin gallate (EGCG/TAA) compared to the TAA group. In addition, histological examination showed a reduction in hepatocyte injury in EGCG/TAA compared to the TAA group. Conclusion: Epigallocatechin gallate administration prior to induction of acute liver injury down-regulates uncoupling protein 2 expression and reduces IL-6, IL-18, TNF-α and CRP.

Original languageEnglish
Pages (from-to)649-654
Number of pages6
JournalInternational Journal of Clinical and Experimental Pathology
Volume8
Issue number1
Publication statusPublished - 1 Jan 2015

Keywords

  • Epigallocatechin gallate
  • Green tea
  • Hepatitis
  • Uncoupling protein 2

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    Jamal, M. H., Mohamed Ali Yaseen, H., Dashti, A., Al-Abbad, J., Dashti, H., Mathew, C., Al-Ali, W., & Asfar, S. (2015). Effect of epigallocatechin gallate on uncoupling protein 2 in acute liver injury. International Journal of Clinical and Experimental Pathology, 8(1), 649-654.