Evaluation of the PrimerDesign™ genesig real-time reverse transcription-polymerase chain reaction assay and the INFINITI® Respiratory Viral Panel Plus assay for the detection of human metapneumovirus in Kuwait

Mariam Al-Turab, Wassim Chehadeh, Fahd Al-Mulla, Widad Al-Nakib

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human metapneumovirus (hMPV) is a respiratory pathogen that was discovered in 2001 and is considered a major cause of both upper and lower respiratory tract infections. A sensitive, fast, and high-throughput diagnostic test is needed for the detection of hMPV that may assist in the clinical management as well as in the reduction of inappropriate therapy. Therefore, a comparison assessment was performed in this study between the PrimerDesign™ genesig real-time reverse transcription-polymerase chain reaction (RT-PCR) Assay and the INFINITI ® Respiratory Viral Panel Plus Assay (RVP-Plus) for the detection of hMPV infection in patients with respiratory tract infections. A total of 200 respiratory samples were collected from 185 hospitalized patients, during the winter season in Kuwait. Of 185 patients, 10 (5.4%) were positive for hMPV RNA by the in-house RT-PCR assay, while 7 (4%) were positive for hMPV RNA by the real-time RT-PCR assay and 9 (5%) were positive for hMPV RNA by the INFINITI ® RVP-Plus assay. The high incidence rate (60%) of hMPV infection was in January 2011. The sensitivity of the real-time RT-PCR and INFINITI ® RVP-Plus assays was 70% and 90%, respectively, with specificity of 100% for both assays. hMPV types A and B could be identified in this study; however, discordant genotyping results were found between the direct sequencing method and the INFINITI ® RVP-Plus assay in 33% of hMPV-positive patients.

Original languageEnglish
Pages (from-to)358-362
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume72
Issue number4
DOIs
Publication statusPublished - 1 Apr 2012

Keywords

  • Human metapneumovirus (hMPV)
  • Microarray
  • Molecular
  • Real-time PCR
  • Respiratory virus
  • RT-PCR

Fingerprint Dive into the research topics of 'Evaluation of the PrimerDesign™ genesig real-time reverse transcription-polymerase chain reaction assay and the INFINITI® Respiratory Viral Panel Plus assay for the detection of human metapneumovirus in Kuwait'. Together they form a unique fingerprint.

  • Cite this