Improving iron absorption from a Peruvian school breakfast meal by adding ascorbic acid or Na2EDTA

Lena Davidsson, Thomas Walczyk, Nelly Zavaleta, Richard F. Hurrell

Research output: Contribution to journalArticlepeer-review

63 Citations (Scopus)


Background: Iron-fortified school breakfasts have been introduced in Peru to combat childhood iron deficiency. Objective: We evaluated whether iron absorption from a school breakfast meal was improved by increasing the ascorbic acid content or by adding an alternative enhancer of iron absorption, Na2EDTA. Design: In a crossover design, iron absorption from test meals was evaluated by erythrocyte incorporation of 58Fe and 57Fe. The test meals (wheat bread and a drink containing cereal, milk, and soy) contained 14 mg added Fe (as ferrous sulfate) including 2.0-2.6 mg 58Fe or 4.0-7.0 mg 57Fe. Results: Geometric mean iron absorption increased significantly from 5.1% to 8.2% after the molar ratio of ascorbic acid to fortification iron was increased from 0.6:1 to 1.6:1 (P < 0.01; n = 9). Geometric mean iron absorption increased significantly from 2.9% to 3.8%, from 2.2% to 3.5%, and from 2.4% to 3.7% after addition of Na2EDTA at molar ratios relative to fortification iron of 0.3:1, 0.7:1, and 1:1, respectively, compared with test meals containing no added enhancers (P < 0.01; n = 10 for all). Iron absorption after addition of ascorbic acid (molar ratio 0.6:1) was not significantly different from that after addition of Na2EDTA (molar ratio 0.7:1). Conclusions: Ascorbic acid and Na2EDTA did not differ significantly in their enhancing effects on iron absorption at molar ratios of 0.6:1 to 0.7:1 relative to fortification iron. Additional ascorbic acid (molar ratio 1.6:1) increased iron absorption significantly. Increasing the molar ratio of Na2EDTA to fortification iron from 0.3:1 to 1:1 had no effect on iron absorption.

Original languageEnglish
Pages (from-to)283-287
Number of pages5
JournalAmerican Journal of Clinical Nutrition
Issue number2
Publication statusPublished - 12 Feb 2001


  • Ascorbic acid
  • Childhood anemia
  • Children
  • Food fortification
  • Iron
  • Iron absorption
  • Iron deficiency
  • Iron fortification
  • NaEDTA
  • Pediatric nutrition
  • Peru
  • Stable isotope
  • Vitamin C


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