Insertion process of a protein single layer within a newton black film

J. J. Benattar, M. Nedyalkov, Ali Tiss, R. Verger, C. Guilbert

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22 Citations (Scopus)


We report a generally applicable protein insertion process leading to the formation of a close packed protein single layer within a freestanding surfactant bilayer. Very high packing fractions can be obtained in a controlled manner, simply by adjusting the protein chemical potential in the solution. We apply this method to the confinement of a model protein in a Newton black film (NBF) of a non-anionic surfactant. Using x-ray reflectivity, we observed a time dependent insertion of the proteins within the NBF and then a stable equilibrium state.

Original languageEnglish
Pages (from-to)5297-5300
Number of pages4
JournalPhysical Review Letters
Issue number26
Publication statusPublished - 1 Jan 1999


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