Abstract
We report a generally applicable protein insertion process leading to the formation of a close packed protein single layer within a freestanding surfactant bilayer. Very high packing fractions can be obtained in a controlled manner, simply by adjusting the protein chemical potential in the solution. We apply this method to the confinement of a model protein in a Newton black film (NBF) of a non-anionic surfactant. Using x-ray reflectivity, we observed a time dependent insertion of the proteins within the NBF and then a stable equilibrium state.
| Original language | English |
|---|---|
| Pages (from-to) | 5297-5300 |
| Number of pages | 4 |
| Journal | Physical Review Letters |
| Volume | 82 |
| Issue number | 26 |
| DOIs | |
| Publication status | Published - 1 Jan 1999 |
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Benattar, J. J., Nedyalkov, M., Tiss, A., Verger, R., & Guilbert, C. (1999). Insertion process of a protein single layer within a newton black film. Physical Review Letters, 82(26), 5297-5300. https://doi.org/10.1103/PhysRevLett.82.5297