Microarray-based CGH and copy number analysis of FFPE samples.

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9 Citations (Scopus)

Abstract

Over the past decade, utilization of microarray technology has flourished in biomedical research. It has evolved rapidly into a revolutionary tool that offers deeper insight into the molecular basis associated with complex diseases, especially in the field of cancer. Specifically, array-based Comparative Genomic Hybridization (aCGH) permits the detection of genome-wide copy number alterations with high resolution. Microarray application to DNA extracted from formalin-fixed paraffin-embedded tissue (FFPE), in particular, poses a challenge due to the partially degraded nature and compromised quality of the DNA. This chapter gives a description of the several CGH-microarray platforms currently available and offers practical steps that guide you through optimal handling and superior aCGH data acquisition of DNA extracted from FFPE tissues.

Original languageEnglish
Pages (from-to)131-145
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume724
DOIs
Publication statusPublished - 15 Jun 2011

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