TY - JOUR
T1 - Seeking sense of antisense switch transcripts
AU - Haddad, Dania
AU - Puget, Nadine
AU - Laviolette-Malirat, Nathalie
AU - Conte, Caroline
AU - Khamlichi, Ahmed Amine
PY - 2011/1/1
Y1 - 2011/1/1
N2 - In B lymphocytes, class switch recombination (CSR) machinery targets highly repetitive sequences, called switch (S) sequences, in the constant domain of the immunoglobulin heavy chain (IgH) locus. Cotranscriptional generation of R loops at S sequences provides the substrate for the mutagenic enzyme AID (Activation-Induced cytidine Deaminase), which initiates the DNA breaks at the transcribed sequences. Both sense and antisense transcripts across the S regions have been reported. Our recent work shows that, unlike its sense counterpart, antisense transcription of S sequences is dispensable for CSR in vivo.
AB - In B lymphocytes, class switch recombination (CSR) machinery targets highly repetitive sequences, called switch (S) sequences, in the constant domain of the immunoglobulin heavy chain (IgH) locus. Cotranscriptional generation of R loops at S sequences provides the substrate for the mutagenic enzyme AID (Activation-Induced cytidine Deaminase), which initiates the DNA breaks at the transcribed sequences. Both sense and antisense transcripts across the S regions have been reported. Our recent work shows that, unlike its sense counterpart, antisense transcription of S sequences is dispensable for CSR in vivo.
KW - AID targeting
KW - Class switch recombination
KW - Sense and antisense transcription
UR - http://www.scopus.com/inward/record.url?scp=79960313216&partnerID=8YFLogxK
U2 - 10.4161/trns.2.4.16784
DO - 10.4161/trns.2.4.16784
M3 - Article
AN - SCOPUS:79960313216
VL - 2
SP - 183
EP - 188
JO - Transcription
JF - Transcription
SN - 2154-1264
IS - 4
ER -