Abstract
Class switch recombination (CSR) occurs between highly repetitive sequences called switch (S) regions and is initiated by activation-induced cytidine deaminase (AID). CSR is preceded by a bidirectional transcription of S regions but the relative importance of sense and antisense transcription for CSR in vivo is unknown. We generated three mouse lines in which we attempted a premature termination of transcriptional elongation by inserting bidirectional transcription terminators upstream of Sγ3 or downstream of Sγ3 sequences. The data show, at least for Sγ3, that sense transcriptional elongation across S region is absolutely required for CSR whereas its antisense counterpart is largely dispensable, strongly suggesting that sense transcription is sufficient for AID targeting to both DNA strands.
Original language | English |
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Pages (from-to) | 1608-1620 |
Number of pages | 13 |
Journal | EMBO Journal |
Volume | 30 |
Issue number | 8 |
DOIs | |
Publication status | Published - 20 Apr 2011 |
Keywords
- AID targeting
- B cell
- class switching
- germ-line transcription