Sense transcription through the S region is essential for immunoglobulin class switch recombination

Dania Haddad, Zéliha Oruc, Nadine Puget, Nathalie Laviolette-Malirat, Magali Philippe, Claire Carrion, Marc Le Bert, Ahmed Amine Khamlichi

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Class switch recombination (CSR) occurs between highly repetitive sequences called switch (S) regions and is initiated by activation-induced cytidine deaminase (AID). CSR is preceded by a bidirectional transcription of S regions but the relative importance of sense and antisense transcription for CSR in vivo is unknown. We generated three mouse lines in which we attempted a premature termination of transcriptional elongation by inserting bidirectional transcription terminators upstream of Sγ3 or downstream of Sγ3 sequences. The data show, at least for Sγ3, that sense transcriptional elongation across S region is absolutely required for CSR whereas its antisense counterpart is largely dispensable, strongly suggesting that sense transcription is sufficient for AID targeting to both DNA strands.

Original languageEnglish
Pages (from-to)1608-1620
Number of pages13
JournalEMBO Journal
Volume30
Issue number8
DOIs
Publication statusPublished - 20 Apr 2011

Keywords

  • AID targeting
  • B cell
  • class switching
  • germ-line transcription

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