Surface behaviour of bile salts and tetrahydrolipstatin at air/water and oil/water interfaces

Ali Tiss, Stéphane Ransac, Hans Lengsfeld, Paul Hadvàry, Alain Cagna, Robert Verger

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47 Citations (Scopus)


The surface behaviour of two bile salts, sodium deoxycholate (NaDC) and sodium taurodeoxycholate (NaTDC), as well as that of tetrahydrolipstatin (THL), a potent gastrointestinal lipase inhibitor, was studied at air/water and oil/water interfaces, using interfacial tensiometry methods. The surface behaviour of NaDC and NaTDC was comparable at both oil/water and air/water interfaces. A fairly compact interfacial monolayer of bile salts is formed well below the critical micellar concentration (CMC) and can help to explain the well-known effects of bile salts on the kinetic behaviour of pancreatic lipases. Using the Wilhelmy plate technique, the surface pressure-molecular area curves recorded with THL at the air/water interface showed a collapse point at a surface pressure of 24.5 mN.m-1, corresponding to a molecular area of 70 Å2. Surprisingly, using the oil drop method, a limiting molecular area of 160 Å2 was found to exist at the oil/water interface. On the basis of the above data, space-filling models were proposed for bile salts and THL at air/water and oil/water interfaces.

Original languageEnglish
Pages (from-to)73-85
Number of pages13
JournalChemistry and Physics of Lipids
Issue number1
Publication statusPublished - 11 Jul 2001


  • Adsorption
  • Bile salt
  • CMC
  • Interface
  • Tetrahydrolipstatin


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