Testosterone enhances cardiomyogenesis in stem cells and recruits the androgen receptor to the MEF2C and HCN4 genes

Ashraf Al Madhoun, Anastassia Voronova, Tammy Ryan, Abeer Zakariyah, Christian McIntire, Laura Gibson, Michael Shelton, Marc Ruel, Ilona S. Skerjanc

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Since a previous study (Goldman-Johnson et al., 2008 [4]) has shown that androgens can stimulate increased differentiation of mouse embryonic stem (mES) cells into cardiomyocytes using a genomic pathway, the aim of our study is to elucidate the molecular mechanisms regulating testosterone-enhanced cardiomyogenesis. Testosterone upregulated cardiomyogenic transcription factors, including GATA4, MEF2C, and Nkx2.5, muscle structural proteins, and the pacemaker ion channel HCN4 in a dose-dependent manner, in mES cells and P19 embryonal carcinoma cells. Knock-down of the androgen receptor (AR) or treatment with anti-androgenic compounds inhibited cardiomyogenesis, supporting the requirement of the genomic pathway. Chromatin immunoprecipitation (ChIP) studies showed that testosterone enhanced recruitment of AR to the regulatory regions of MEF2C and HCN4 genes, which was associated with increased histone acetylation. In summary, testosterone upregulated cardiomyogenic transcription factor and HCN4 expression in stem cells. Further, testosterone induced cardiomyogenesis, at least in part, by recruiting the AR receptor to the regulatory regions of the MEF2C and HCN4 genes. These results provide a detailed molecular analysis of the function of testosterone in stem cells and may offer molecular insight into the role of steroids in the heart.

Original languageEnglish
Pages (from-to)164-171
Number of pages8
JournalJournal of Molecular and Cellular Cardiology
Volume60
Issue number1
DOIs
Publication statusPublished - 1 Jul 2013

Keywords

  • Androgen receptor
  • Cardiomyogenesis
  • Gene expression
  • HCN4
  • MEF2
  • Testosterone

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